phosphorylated p p65 rabbit polyclonal antibody  (Bioss)

Journal: Journal of Neuroinflammation

Article Title: Tanshinone IIA alleviates brain damage in a mouse model of neuromyelitis optica spectrum disorder by inducing neutrophil apoptosis

doi: 10.1186/s12974-020-01874-6

Figure Lengend Snippet: TSA suppressed activation of the NF-κB signaling pathway. Western blotting was performed to analyze the levels of p-p65, t-p65, p-p38, t-p38, p-Erk1/2, and t-Erk1/2 in the hemispheres containing the lesions from TSA- or vehicle-treated NMOSD mice at the end of the experiment. a Representative western blots showing p-p65, t-p65, p-p38, t-p38, p-Erk1/2, t-Erk1/2, and β-actin. b Quantification of the expression of p-p65 relative to t-p65. c Quantification of the expression of p-p38 relative to t-p38. d Quantification of the expression of p-Erk relative to t-Erk ( n = 5). β-actin was used as an internal control. n = 5, * p < 0.05, *** p < 0.001. One-way ANOVA followed by Tukey’s multiple comparison tests was performed

Article Snippet: Then, the membranes were washed twice with Tris-buffered saline with 0.05% Tween-20 (TBST) and blocked with 5% skim milk (Difco) for 1 h at room temperature followed by incubation with rabbit anti-phosphorylated p65 (p-p65) polyclonal antibody (1:1000, Cell Signaling Technology, catalog 3033 T), rabbit anti-total p65 (t-p65) monoclonal antibody (1:1000, Cell Signaling Technology, catalog 8242 T), rabbit anti-phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) monoclonal antibody (1:1000, Cell Signaling Technology, catalog 9211S), rabbit anti-total p38 MAPK (t-p38 MAPK) monoclonal antibody (1:1000, Cell Signaling Technology, catalog 9212S), rabbit anti-phosphorylated extracellular-regulated protein kinases 1/2 (p-Erk1/2) monoclonal antibody (1:1000, Cell Signaling Technology, catalog 4377), rabbit anti-total Erk1/2 (t-Erk1/2) polyclonal antibody (1:1000, Cell Signaling Technology, catalog 9102), rabbit anti-caspase-3 polyclonal antibody (1:1000, Proteintech, catalog 19677-1-AP), and rabbit anti-β-actin polyclonal antibody (1:1000, Bioss, catalog: bs-0061R) overnight at 4 °C.

Techniques: Activation Assay, Western Blot, Expressing, Control, Comparison